Selections can be moved by clicking and dragging. The length of line selections can be measured using Analyze/Measure. Use Edit/Draw to draw the line in the current color. Line selections are created using the straight, segmented and freehand line selection tools. Area selections can be measured ( Analyze/Measure), filtered, filled ( Edit/Fill) or drawn ( Edit/Draw). Area selections are created using the rectangular, oval, polygonal and freehand selection tools. Only one selection can be active at a time. Selections are user defined areas or lines within an image. The Image/Stacks submenu contains commands for common "Slices" field to a value greater than one. To create a new stack, simply choose File/New and set the The File/Acquire/Raw command opens other multi-image, uncompressed files. ImageJ opens multi-image TIFF files as a stack, and saves stacks as multi-image TIFFs. ImageJ filters will process all the slices in a stack. A scroll bar provides the ability to move through the slices. All the slices in a stack mustīe the same size and bit depth. The images that make up a stack are called slices. ImageJ can display multiple spatially or temporally related images in a single window. The window is defined by minimum and maximum values that can be modified using Image/Adjust/Brightness/Contrast. The window defines the range of gray values that are displayed: values below the window are made black, while values above the window are white. Therefore, the data are mapped to 8-bits by windowing. 16-bit images use unsigned integers (0 to 65,535) and 32-bit grayscale images use floating-point numbers.ġ6-bit and 32-bit grayscale images are not directly displayable on computer monitors, which typically can show only 256 shades of gray. 8-bit images are represented using unsigned integers in the range 0 to 255. ImageJ supports 8-bit, 16-bit and 32-bit (real) grayscale images and 8-bit and 32-bit color images. All operations will be performed on the active image. The active window has its title bar highlighted. ImageJ allows multiple images to be displayed on the screen at one time. It will not appear if the operation requires less then approximately one second. The progress bar, located to the right of the status bar, shows the progress of time-consuming operations. After running a filter, it displays the elapsed time and processing rate in pixels/second. The status bar, when the cursor is over an image, displays pixel coordinates and values. Click on a tool and a description of that tool is displayed in the status bar. The tool bar contains tools for making selections, for zooming and scrolling images, and for changing the drawing color. Histograms and plots are ordinary image windows that can be copied (to the internal clipboard), edited, printed and saved. Windows can be dragged around the screen and resized. Measurement results are displayed in the "Results" window. The "ImageJ" window contains a menu bar (at the top of the screen on the Mac), tool bar, status bar, and a progress bar. Home | contents | previous | next Basic Concepts
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